Coding

Part:BBa_K801070:Design

Designed by: Roman Prechtl   Group: iGEM12_TU_Munich   (2012-09-21)

xanthosine methyltransferase CaXMT1-strep


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]




synthesized by IDT, yeast codon optimized


Keywords: koffein, coffein, caffeine, xanthosine, coffea, arabica


Abbreviations: CaXMT1 = coffea arabica xanthosine N-methyltransferase 1

Design Notes

Related BioBrick: BBa_K801073 BBa_K801076 BBa_K801077

Quality control measures:

  • Test digest has been performed with use of Xba1 and Pst1 (see mainpage)
  • Part has been totally sequenced


Backbone:

  • Backbone name: pSB1C3
  • Resistance: Cp

Protein coding:

  • Protein: CaXMT1-Strep-tag

Enzymatic activity:

EC-number [http://enzyme.expasy.org/EC/2.1.1.158 2.1.1.158]

Cytotoxicity:
cytotoxic for S.cervisiaegrowth inhibition at 10 mM, lethal effect at 100 mM

Safety notes:

Intellectual property:

Corresponding part author/authors:

Source

Source:
synthesized by IDT


Organism:

  • Genesequence derived from coffea arabica
  • codonoptimized for saccharomyces cerevisiae
  • Designed for the following Chassis: saccharomyces cerevisiae


References

Literature references:
Uefuji, H., Ogita, S., Yamaguchi, Y., Koizumi, N., and Sano, H. (2003). Molecular cloning and functional characterization of three distinct n-methyltransferases involved in the caffeine biosynthetic pathway in coffee plants. Plant Physiol, 132(1):372–80.

Database references:

  • NCBI: [http://www.ncbi.nlm.nih.gov/nuccore/AB048794.1 Coffea arabica CaMXMT1 mRNA for 7-methylxanthine N-methyltransferase, complete cds]
  • uniprot: [http://www.uniprot.org/uniprot/q9avk0 Q9AVK0 (XMT1_COFAR)]